BIOL 217 Molecular Genetics & Protein Engineering
This laboratory course takes the students through a connected series of modern and advanced molecular biology concepts such as sequence and structural analysis of a model gene, introduction of a specific mutation in a key functional motif, and biochemical assays to determine how the mutation affects biological function. The drug resistance ABC transporter gene, pdr5, from Saccharomyces cerevisiae, a gene that has significant biomedical importance, will be used as the model gene. The course builds on concepts learned in Biol. 106 laboratory by incorporating several new approaches such as computational analysis, protein engineering, site-directed mutagenesis, and enzyme biochemistry. A major goal of this course is to provide the students with skills that are inherent in professional success, which include analytical skills, quantitative and statistical analyses, written and oral communication of basic concepts.
FOR BIOLOGY and MED TECH MAJORS ONLY.